蛋白质聚集的三种途径和控制策略

Three ways for protein aggregation and the control strategies

摘要:

蛋白质聚集在生物医药生产中是一个关键问题。在蛋白质的生产、运输和储存的过程中，多种因素都能导致蛋白质发生聚集。随着对蛋白质聚集这一现象的深入研究，发现蛋白质聚集的产生存在不同途径和各种影响因素，如理化因素、翻译修饰和蛋白质结构等。由于蛋白质的聚集对于蛋白质的活性和均一性具有重大影响，因此了解蛋白质聚集的途径以及研究如何控制聚集对获得均质蛋白是十分有意义的。本文主要阐述了3D结构域交换、盐桥的形成、氧化应激3种蛋白质的聚集途径，以及在蛋白质生产、运输、储存过程中控制蛋白质聚集的方法，这有助于减少由于蛋白质聚集体形成而造成的损失，并提高实验研究和商业生产中的蛋白质纯度和均质性。

Abstract:

Protein aggregation is a critical issue in the production of biopharmaceuticals. During protein production, transport and storage, various factors can lead to protein aggregation. With the in-depth study, different ways of protein aggregation and various influencing factors were identified. This includes physical and chemical factors, translation modifications and protein structure. Since protein aggregation exerts major impact on the activity and homogeneity of proteins, it is of great importance to study the ways of protein aggregation and how to control it to obtain high-quality proteins. The review focuses on three ways of protein aggregation, namely 3D domain swapping, salt bridge formation, and oxidative stress, as well as methods to control protein aggregation during protein production, transport and storage. This may facilitate reducing the loss caused by the formation of protein aggregation and improving the purity and homogeneity of protein in research and commercial production.