生长抑素受体2的小鼠时空组织表达谱

Spatial and temporal expression pattern of somatostatin receptor 2 in mouse

摘要:

生长抑素（somatostatin，SST）作为一种抑制性多肽激素，在多种生物过程中发挥重要的功能。生长抑素受体2（somatostatin receptor 2，SSTR2）作为生长抑素表达最广泛的受体在多种组织中表达，但其表达的具体细胞类型尚不清楚。本研究在小鼠不同发育阶段的多种组织中鉴定了SSTR2蛋白表达的细胞类型。通过多色免疫荧光在小鼠胚胎期15.5 d、出生后1 d、7 d、15 d、3个月和6个月的脑、骨、肺、肠道、皮肤、胃、脾和肾等组织中检测了Sstr2基因的表达。结果发现Sstr2在不同发育阶段的多种组织的特定细胞类型中表达，包括脑神经元和星形胶质细胞，骨的间充质基质细胞、造血细胞和B细胞，肺的巨噬细胞、II型肺泡上皮细胞和气道纤毛细胞，肠道的上皮细胞和神经元，皮肤的毛囊细胞，胃体的上皮细胞，脾的造血干细胞、造血祖细胞和神经纤维，肾的肾小管上皮细胞等。本研究确定了小鼠多组织不同发育阶段Sstr2表达的细胞类型，为生长抑素与生长抑素受体2的生理功能研究提供了新的线索。

Abstract:

Somatostatin (SST) is an inhibitory polypeptide hormone that plays an important role in a variety of biological processes. Somatostatin receptor 2 (SSTR2) is the most widely expressed somatostatin receptor. However, the specific cell types expressing Sstr2 in the tissues have not been investigated. In this study, we detected the expression pattern of SSTR2 protein in mouse at different development stages, including the embryonic 15.5 days and the postnatal 1, 7, 15 days as well as 3 and 6 months, by multicolour immunofluorescence analyses. We found that Sstr2 was expressed in some specific cells types of several tissues, including the neuronal cells and astrocytes in the brain, the mesenchymal cells, the hematopoietic cells, the early hematopoietic stem cells, and the B cells in the bone marrow, the macrophages, the type II alveolar epithelial cells, and the airway ciliated cells in the lung, the epithelial cells and the neuronal cells in the intestine, the hair follicle cells, the gastric epithelial cells, the hematopoietic stem cells and the nerve fibre in the spleen, and the tubular epithelial cells in the kidney. This study identified the specific cell types expressing Sstr2 in mouse at different developmental stages, providing new insights into the physiological function of SST and SSTR2 in several cell types.