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2025年4月5日 21:36 星期六
首页 > 过刊浏览>2023年第39卷第7期 >2762-2771. DOI:10.13345/j.cjb.220850
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大豆GmGolS2-2提高转基因烟草耐旱性
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黑龙江省省属高等学校基本科研业务费科研项目（145109506）；国家自然科学基金（32101694）


Soybean GmGolS2-2 improves drought resistance of transgenic tobacco
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    摘要:

    肌醇半乳糖苷合成酶（galactinol synthase，GolS）基因在植物应对非生物胁迫时发挥重要作用。本研究构建大豆GmGolS2-2基因植物表达载体并转化烟草，对转基因烟草的耐旱性进行鉴定。通过逆转录-聚合酶链式反应（reverse transcription-polymerase chain reaction，RT-PCR）从大豆叶片中克隆了975 bp的GmGolS2-2基因编码序列。通过限制性内切酶位点Nde I和EcoR I将GmGolS2-2基因与植物表达载体pRI101相连，通过叶盘法转化烟草。基因组DNA PCR和实时荧光定量PCR结果显示，共获得3棵GmGolS2-2转基因烟草。干旱胁迫下GmGolS2-2转基因烟草的生长状态好于野生型烟草。干旱胁迫处理后，转基因烟草的电解质渗透率和丙二醛含量低于野生型烟草，脯氨酸含量和可溶性糖含量则高于野生型烟草。实时荧光定量PCR结果显示，GmGolS2-2的异源表达提高了转基因烟草中抗逆相关基因NtERD10C和NtAQP1的表达量。以上结果表明，GmGolS2-2提高了转基因烟草的耐旱性。

    Abstract:

    Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean GmGolS2-2 gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A GmGolS2-2 gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). GmGolS2-2 was linked to the plant expression vector pRI101 by restriction enzyme sites Nde I and EcoR I, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three GmGolS2-2 transgenic tobacco plants were obtained. The growth status of GmGolS2-2 transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of GmGolS2-2 increased the expression of stress-related genes NtERD10C and NtAQP1 in transgenic tobacco. The above results indicated that GmGolS2-2 improved drought resistance of transgenic tobacco.

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于海伟,邱爽,张军,李珊珊,孙天国,马天意,赵艳,赵旭,翟莹. 大豆GmGolS2-2提高转基因烟草耐旱性[J]. 生物工程学报, 2023, 39(7): 2762-2771

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  • 收稿日期:2022-10-24
  • 录用日期:2023-01-10
  • 在线发布日期: 2023-07-11
  • 出版日期: 2022-07-25
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