不同粒径口蹄疫病毒样颗粒-ZIF-8复合物诱导小鼠的体液免疫效果分析

doi:10.13345/j.cjb.230070

科微学术

生物工程学报

首页 > 过刊浏览>2023年第39卷第12期 >4837-4848. DOI:10.13345/j.cjb.230070

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不同粒径口蹄疫病毒样颗粒-ZIF-8复合物诱导小鼠的体液免疫效果分析
DOI:
                        10.13345/j.cjb.230070
                    
CSTR:
                        32114.14.j.cjb.230070
                    
作者:
                        李家俊李家俊
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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王俊王俊
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046;黑龙江八一农垦大学动物科技学院, 黑龙江 大庆 163319
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张韵张韵
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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滕志东滕志东
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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董虎董虎
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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郭慧琛郭慧琛
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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孙世琪孙世琪
中国农业科学院兰州兽医研究所 动物疫病防控全国重点实验室 口蹄疫国家参考实验室, 甘肃 兰州 730046
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基金项目:国家重点研发计划(2021YFD1800300)；国家自然科学基金(32072859，32072847，32002272)；甘肃省科技重大专项计划(21ZD3NA001)；科技人才与平台计划(202205AF150007)

Evaluation of the humoral immunity in mice induced by foot-and-mouth disease virus-like particles-ZIF-8 complexes with different sizes

Author:

LI Jiajun
LI Jiajun
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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WANG Jun
WANG Jun
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China;College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
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ZHANG Yun
ZHANG Yun
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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TENG Zhidong
TENG Zhidong
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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DONG Hu
DONG Hu
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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GUO Huichen
GUO Huichen
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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SUN Shiqi
SUN Shiqi
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
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摘要:

为进一步提高口蹄疫(foot-and-mouth disease, FMD)病毒样颗粒(virus-like particles, VLPs)疫苗的免疫效果，本研究采用仿生矿化方法，将Zn²⁺和2-甲基咪唑按照不同浓度配比制备了不同粒径的FMDV VLPs-沸石咪唑骨架-8 (zeolitic imidazolate framework-8, ZIF-8)复合物，以探究尺寸效应对免疫效果的影响。结果显示，成功制备出3种不同粒径的FMDV VLPs-ZIF-8，粒径分别约为70、100、1 000 nm。细胞毒性和组织病理学试验表明，3种复合物均具有良好的生物安全性。小鼠免疫试验表明，3种复合物均能明显提高中和抗体和特异性抗体水平，并且随着复合物体积的减小，其免疫效果也随之增强。本研究表明，ZIF-8包封FMDV VLPs可显著增强其免疫效果，且具有尺寸依赖性。

关键词:沸石咪唑骨架-8;口蹄疫病毒样颗粒;佐剂;尺寸效应

Abstract:

To further enhance the immune effect of the foot-and-mouth disease (FMD) virus-like particles (VLPs) vaccine, this study prepared FMDV VLPs-zeolitic imidazolate (framework-8, ZIF-8) complexes with different particle sizes. We used a biomimetic mineralization method with Zn²⁺ and 2-methylimidazole in different concentration ratios to investigate the effect of size on the immunization effect. The results showed that FMDV VLPs-ZIF-8 with three different sizes were successfully prepared, with an approximate size of 70 nm, 100 nm, and 1 000 nm, respectively. Cytotoxicity and animal toxicity tests showed that all three complexes exhibited excellent biological safety. Immunization tests in mice showed that all three complexes enhanced the titers of neutralizing and specific antibodies, and their immune effects improved as the size of the complexes decreased. This study showed that ZIF-8 encapsulation of FMDV VLPs significantly enhanced their immunogenic effect in a size-dependent manner.

Key words:zeolitic imidazolate framework-8 (ZIF-8);foot-and-mouth disease virus-like particles (FMDV VLPs);adjuvants;size effect

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