Abstract:Under the background of the "era of mass innovation", there are challenges in the training of biotechnology professionals, including a "backward concept of innovation and entrepreneurship education", a "singular education method of innovation and entrepreneurship", and a "limited practice platform of innovation and entrepreneurship". These challenges require the implementation of a new training model. In comparison to the talent training objectives of new engineering construction, the College of Biotechnology and Bioengineering at Zhejiang University of Technology has been exploring and practicing the training mode "tri-bio, tri-chain and tri-creation " for 42 years. The research has established a new platform and paradigm for training exceptional engineering innovation and entrepreneurship talents. It also offers valuable references and insights for the reform of training methods for biotechnology professionals by optimizing the education concept of "biology, life and live ", enriching the education method of "knowledge chain, scientific research chain and industrial chain", and building the three-creation technology practice platform based on "creativity, innovation and entrepreneurship".