CRISPR/Cas9基因编辑技术在食用菌中的应用现状

Application of CRISPR/Cas9 gene editing technology in edible fungi: a review

摘要:

CRISPR/Cas9基因编辑系统是一项通用的基因修饰技术，是植物、动物、真菌以及微生物的功能基因和遗传育种研究的重要技术。本文介绍了该技术在食用真菌的基因研究和遗传育种中的应用现状，包括Cas9和sgRNA的递送和表达策略、遗传转化方法、突变体筛选以及DNA双链断裂后的靶位点的修复策略。同时总结了该技术在食用菌中应用所面临的主要问题及其优化策略，并结合个人研究背景展望了其未来在食用菌研究中的应用价值。

Abstract:

The CRISPR/Cas9 gene editing system is a versatile technology for modifying gene, playing a crucial role in the study of functional genes and genetic breeding of plants, animals, fungi, and microorganisms. This review provides a comprehensive analysis of the application of this technology in gene research and genetic breeding of edible fungi. The review covers various aspects, including the delivery and expression strategies of Cas9 and sgRNA, genetic transformation methods, mutant screening, and repair strategies for target sites following DNA double-strand breaks. Additionally, the review summarizes the main challenges and optimization strategies associated with the application of this technology in edible fungi. Lastly, the future application potential of this technology in edible fungi research is discussed, drawing from the authors’ personal research background.