CRISPR/Cas9基因编辑技术水稻育种应用的实验教学设计

Experimental teaching design of CRISPR/Cas9 technology in rice breeding application

摘要:

CRISPR/Cas9基因编辑技术在作物育种中已具有重要的应用价值，理解与掌握该项技术能够为生物学、农学等专业的学生从事相关科研工作打好基础。为了将CRISPR/Cas9技术融入高校的实验教学中，设计开发了一个创新性教学实验“利用CRISPR/Cas9技术提高水稻植株的白叶枯病抗性”。该实验有助于学生更好地理解CRISPR/Cas9技术原理，掌握其操作流程和方法，学习应用该技术对水稻进行定向分子育种改良。在拓展学生知识和技能的同时，推进实验教学的改革与创新。

Abstract:

The CRISPR/Cas9 gene editing technology has proven to be valuable in crop breeding applications. Understanding and mastering this technology will provide a strong foundation for students majoring in biology, agronomy, and related fields to engage in scientific research and work. To incorporate CRISPR/Cas9 technology into experimental teaching courses at colleges, an innovative teaching experiment entitled “Enhancing the resistance of rice plants to bacterial blight disease using CRISPR/Cas9 technology” was designed. The experiment allows students to deepen their understanding of the basic principles of CRISPR/Cas technology, acquire proficiency in its protocol, and learn to apply the technology for targeted molecular breeding of rice. It not only expands students’ knowledge and skills, but also promotes the reform and innovation of experimental teaching methods.