不同尺寸形貌抑冰微纳米材料对细胞冷冻保护性能的影响

Cryoprotective effects of deicing micro/nanomaterials with different sizes and shapes on cells

摘要:

抑冰纳米材料被广泛研究以提高对细胞、组织或器官等的冷冻保存效率。不同组分、尺寸、形貌的抑冰纳米材料可有效抑制冰晶的形成和生长，从而减少对生物样品冻存的损伤。本研究通过水热法制备出不同尺寸的碳复合体微粒(carbon composite particles, CCPs)和特殊形貌的微纳米材料。结果显示随着碳复合体微粒粒径尺寸减小，细胞冻存保护效果增强。而相较于球形结构的碳复合体微粒，不对称纳米粒子(Janus nanoparticles)和纳米花(WSP nanoflower)材料对细胞冻存的保护作用明显增强。另外，将适当浓度微纳米抑冰材料与商品化冷冻保护剂联合使用，可进一步提高对细胞冷冻保护的效果。本文制备的微纳米防冻材料可有效提高细胞的冷冻保存效率，在生物医学研究和低温保存应用中具有巨大的潜力。

Abstract:

Extensive studies have been conducted on deicing nanomaterials to improve the cryoprotective effects on cells, tissues, and organs. The nanomaterials with different composition, sizes, and shapes can inhibit the formation and growth of ice crystals, thereby reducing the damage to the cryopreserved samples. In this study, the carbon composite particles (CCPs) with different sizes and shapes were prepared by the hydrothermal method. The results demonstrated that the cryoprotective effect of CCPs enhanced with the decrease in particle size. Compared with spherical CCPs, Janus nanoparticles and WSP nanoflower with special shapes demonstrated improved protective effects on cryopreserved cells. In addition, the combination of deicing micro/nanomaterials at appropriate concentrations with commercial cryoprotectants exerted improved cryoprotective effects on cells. The prepared deicing micro/nanomaterials can improve cell cryopreservation, demonstrating great application potential in biomedical research and cryopreservation.