水稻籽粒VB6含量的调控位点挖掘及分析

Mining and analysis of regulatory loci for VB6 content in rice grains

摘要:

维生素B6(vitamin B6,VB6)作为参与动植物多项生命活动的重要组成成分，体现了水稻等粮食经济作物的营养品质。目前对于控制水稻籽粒VB6含量的相关基因的挖掘较少且不深入。本研究以‘华占(HZ)’和‘热研2号(Nekken2)’为亲本所构建的重组自交系为材料，在数量性状基因座(quantitative trait loci,QTL)定位的基础上进行候选基因初步筛选，共获得10个候选基因，其中LOC_Os01g52450、LOC_Os01g52500、LOC_Os05g09500、LOC_Os05g09440、LOC_Os05g20570和LOC_Os05g36270基因的表达量在双亲间差异显著。结合基因表达量和亲本VB6含量数据，推测LOC_Os05g09500是影响水稻籽粒VB6含量的关键基因，其高表达显著影响水稻籽粒VB6含量。本研究填补了水稻籽粒VB6性状QTL定位研究的空白，为进一步阐明水稻VB6合成的分子遗传机理、克隆相关基因提供了理论支持，在鉴定、筛选和培育高VB6含量的水稻新品种方面具有一定意义。

关键词:水稻;维生素B6;数量性状基因座定位;营养品质

Abstract:

Vitamin B6 (VB6), as an essential component involved in numerous biological activities of animals and plants, reflects the nutritional quality of cereal crops such as rice. Few studies have been conducted to mine the genes controlling the VB6 content in rice grains, and the available studies remain to be deepened. In this study, the recombinant inbred lines created from parents ‘HZ’ and ‘Nekken2’ served as the experimental materials. Based on QTL mapping, the initial screening identified ten candidate genes. The expression levels of LOC_Os01g52450, LOC_Os01g52500, LOC_Os05g09500, LOC_Os05g09440, LOC_Os05g20570, and LOC_Os05g36270 showed significant differences between the parents. According to the gene expression and parental VB6 content, we hypothesized LOC_Os05g09500 as the key gene affecting the VB6 content in rice grains, and the high expression of this gene significantly influenced the VB6 content. The results of this study fill a gap in the QTL mapping on the VB6 content of rice grains and provide theoretical support for elucidating the molecular genetic mechanisms and cloning the related genes of VB6 synthesis in rice. In addition, the findings have significant implications for identifying, screening, and breeding new rice cultivars with high VB6 content.

Key words:rice;vitamin B6;quantitative trait loci (QTL) localization;nutritional quality