微生物转录组学技术研究进展

Advances in microbial transcriptomics techniques

摘要:

随着分子生物技术的不断进步，转录组学技术已广泛应用于生物基因表达水平的研究。近年来，针对微生物转录组学的研究技术也在不断发展。在基因层面上，由片段RNA与微生物样本进行互补验证，发展到直接对全长RNA进行测序得到序列信息；在空间上，由传统群体转录组发展到空间、单细胞以及表观等层面的研究。随着转录组学技术在微生物研究领域中的应用，相应的缺陷也逐渐显现并不断被完善。本文主要是对微生物研究方面传统的和新型的转录组学技术进行了总结归纳，为微生物转录组学研究提供参考。

Abstract:

With the rapid development of molecular biotechnology, transcriptomics has been widely used in the study of gene expression. In recent years, the techniques for microbial transcriptomics research have also been rapidly developing. At the gene level, the way for obtaining sequence information has been developed from complementary validation of RNA fragment through DNA microarray to direct sequencing of full-length RNA. Spatially, the traditional population transcriptomics technique has been developed into spatial, single cell and epigenetic transcriptomics studies. With the application of transcriptomics techniques in the field of microbial research, the corresponding defects were gradually revealed and constantly improved. In this paper, the traditional and new transcriptomics techniques in the field of microbial research are summarized to provide reference for microbial transcriptomics research.