大鼠急性低氧模型尿液蛋白质组的变化

Changes in the urine proteome in an acute hypoxic rat model

摘要:

本研究旨在探究急性低氧对大鼠尿液蛋白质组造成的影响。在该项研究中，大鼠被放置于模拟海拔5 000 m高原环境的低氧舱内24 h。在低氧后0、12、24 h收集尿液样本，并使用液相色谱-串联质谱技术（LC-MS/MS）对尿蛋白进行分析。与低氧0 h相比，低氧12 h组共鉴定到144个差异蛋白，低氧24 h组共鉴定到129个差异蛋白。功能分析显示，差异蛋白参与了一系列与低氧应激有关的生物学通路，如抗氧化应激、糖酵解、补体和凝血级联反应等。研究结果表明，尿液蛋白质组可以反映急性低氧刺激后的显著变化。这些发现可能提供了一种判断机体缺氧状态的方法，有助于辅助检测缺氧状态。

Abstract:

The purpose of this study was to explore the effect of acute hypoxia on urine proteome in rats. In this study, rats were placed in a hypoxic chamber simulating a plateau environment at an altitude of 5 000 m for 24 hours. Urine samples were collected at 0, 12, and 24 h after hypoxia. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (before hypoxia), a total of 144 differentially expressed proteins were identified in the hypoxia 12 h group, and 129 differentially expressed proteins were identified in the hypoxia 24 h group. Functional annotation analysis revealed that these differentially expressed proteins were involved in a series of biological pathways related to hypoxic stress, such as anti-oxidative stress, glycolysis, complement and coagulation cascade. Our results suggest that the urinary proteome can reflect significant changes upon acute hypoxic stimulation. These findings may provide an approach to judge the hypoxia state of the body and help to assist the detection of hypoxia state.