植物源二萜类化合物微生物合成研究进展

Advances on the microbial synthesis of plant-derived diterpenoids

摘要:

植物源二萜类天然产物结构复杂且功能多样，具有抗癌、抗炎和抗菌等多种药理活性，在药品、化妆品和食品添加剂等方面广泛应用。近年来，基于植物源二萜类化合物(diterpenoids)生物合成途径中功能基因的逐步揭示和合成生物技术的发展，科研人员采用代谢工程技术构建了多种二萜类化合物的微生物细胞工厂，且多个化合物达到克级产量。本文对植物源二萜类化合物微生物细胞工厂的构建情况进行综述，介绍并探讨植物源二萜类化合物微生物合成的研究进展和改造策略，为高产二萜类化合物细胞工厂构建和工业化生产提供参考。

Abstract:

Natural plant-derived diterpenoids are a class of compounds with diverse structures and functions. These compounds are widely used in pharmaceuticals, cosmetics and food additives industries because of their pharmacological properties such as anticancer, anti-inflammatory and antibacterial activities. In recent years, with the gradual discovery of functional genes in the biosynthetic pathway of plant-derived diterpenoids and the development of synthetic biotechnology, great efforts have been made to construct a variety of diterpenoid microbial cell factories through metabolic engineering and synthetic biology, resulting in gram-level production of many compounds. This article summarizes the construction of plant-derived diterpenoid microbial cell factories through synthetic biotechnology, followed by introducing the metabolic engineering strategies applied to improve plant-derived diterpenoids production, with the aim to provide a reference for the construction of high-yield plant-derived diterpenoid microbial cell factories and the industrial production of diterpenoids.