全细胞催化合成2,5-呋喃二甲酸

Whole-cell catalytic synthesis of 2,5-furandicarboxylic acid

摘要:

2,5-呋喃二甲酸(2,5-furandicarboxylic acid, FDCA)作为最具代表性的平台化合物，可用于替代石油基的对苯二甲酸，在可降解塑料等领域有着广泛的应用前景。传统的热催化和电化学催化法生产FDCA存在催化剂稳定性差、需要强碱环境等问题；而生物催化反应条件温和，但截至目前相关研究较少，同时生物法转化率和生产强度相对较低。为探索FDCA的生物合成路径，提高生物催化剂的稳定性与FDCA的合成效率，构建绿色低碳的生产体系，本研究以2,5-二甲酰基呋喃(furan-2,5-dicarbaldehyde, DFF)为底物，筛选得到1株高产FDCA的乌鲁木齐奇异球菌(Deinococcus wulumuqiensis) DWCS-2，通过限制性因素分析和过程强化，在摇瓶水平成功将10 g/L DFF完全催化为12.5 g/L FDCA。随后将催化体系扩大至5 L发酵罐，通过分批次补加底物，FDCA产量最高达到18.8 g/L，转化率达到99%。本研究有利于进一步推进FDCA的生物法合成。

Abstract:

As one of the most representative platform compounds, 2,5-furandicarboxylic acid (FDCA) offers significant potential as a renewable alternative to petroleum-derived terephthalic acid, particularly in the field of biodegradable plastics. Traditional thermocatalytic and electrochemical methods for FDCA production face challenges such as poor catalyst stability and the requirement for strong alkaline conditions. In contrast, biocatalytic approaches operate under milder conditions. However, relevant studies remain limited, and biocatalytic processes often exhibit relatively low conversion rates and production intensity. To explore the biosynthetic pathway of FDCA, enhance the stability of biocatalysts and the synthesis efficiency of FDCA, thereby establishing a green and low-carbon production system. In this study, using 2,5-diformylfuran (DFF) as the substrate, a strain of Deinococcus wulumuqiensis DWCS-2 capable of high-yield FDCA production was isolated. Through restrictive factor analysis and process intensification, 10 g/L of DFF was completely converted to 12.5 g/L of FDCA at the shake-flask level. Subsequently, the catalytic system was scaled up to a 5 L fermenter. By employing a fed-batch substrate supplementation strategy, the FDCA titer reached a maximum of 18.8 g/L with a conversion rate of 99%, representing the highest yield reported for whole-cell production of FDCA from DFF to date. This study contributes to advancing the biosynthesis of 2,5-furandicarboxylic acid.