白腐真菌Pleurotus sajor-caju预处理对红麻秸秆发酵乙醇的影响

Effects of microbial pretreatment of kenaf stalk by the white-rot fungus Pleurotus sajor-caju on bioconversion of fuel ethanol production

摘要:

为研究微生物法预处理对红麻秸秆中木质素的降解及后续的红麻纤维素酶促糖化和发酵效率的影响，将白腐真菌Pleurotus sajor-caju接种在红麻秸秆培养基上固态培养，对红麻秸秆进行预处理。经P. sajor-caju培养25~35 d后，有效转化红麻秸秆中的木质素，转化率最高可达50.20％，并提高红麻纤维素的酶促水解效率，糖化率达69.33％~78.64％，与对照组相比提高了3.5~4.1倍。以微生物法预处理后的红麻秸秆样品为底物的同步糖化发酵实验表明，发酵72 h，发酵液中乙醇浓度达到18.35~

Abstract:

Kenaf stalk was pretreated by the white-rot fungus Pleurotus sajor-caju incubated in solid-state kenaf stalk cultivation medium. Delignification and subsequent enzymatic saccharification and fermentation of kenaf stalk were investigated in order to evaluate effects of microbial pretreatment on bioconversion of kenaf lignocellulose to fuel ethanol production. The highest delignification rate of 50.20% was obtained after 25?35 days cultivation by P. sajor-caju, which could improve subsequent enzymatic hydrolysis efficiency of kenaf cellulose. And the saccharification rate of pretreated kenaf stalk reached 69.33 to 78.64%, 4.5?5.1 times higher than the control. Simultaneous saccharification and fermentation (SSF) with microbial-pretreatment kenaf stalk as substrate was performed. The highest overall ethanol yield of 68.31% with 18.35 to 18.90 mg/mL was achieved after 72 h of SSF.