鸡GM-CSF和IL-2增强表达新城疫病毒F蛋白的重组杆状病毒疫苗的免疫效果

Efficacy enhancement of a Baculovirus-vectored Newcastle Disease Virus F protein vaccine by chicken GM-CSF and IL-2

摘要:

为比较鸡粒细胞-巨噬细胞集落刺激因子 (Granulocyte macrophage colony stimulating factor，GM-CSF) 及鸡白细胞介素2 (Interleukin 2，IL-2) 对杆状病毒疫苗的免疫增强效果，通过基因工程手段构建重组杆状病毒疫苗 (Recombinant Baculovirus，BV) rBV-LMI-F，并联合GM-CSF及IL-2进行鸡体免疫。对中和抗体水平及细胞因子含量比较GM-CSF和IL-2的免疫增强效果进行对比。结果显示，在第一次免疫28 d或42 d后，GM-CSF联合免疫组可诱导鸡体产生更高的抗体和细胞因子水平 (P<0.01)。表明鸡GM-CSF能更有效地刺激机体产生较强的抗体和细胞因子反应，提高重组杆状病毒疫苗的免疫效果。

Abstract:

To compare with the effects of the GM-CSF and IL-2 used as adjuvants in the baculovirus vaccine, we used genetic engineering to construct the recombinant baculovirus rBV-LMI-F and with GM-CSF and IL-2 to immunized chickens. Then, we compared the concentration of the neutralizing antibody and cytokines to determine the immunostimulatory effects of GM-CSF and IL-2. GM-CSF induced higher levels of antibodies and cytokines in chickens at 28 d and 42 d post-vaccination. In conclusion, GM-CSF could elicit higher serum antibody and cytokines responses and improved the effects of Baculovirus vaccine.