昆虫杆状病毒表达载体系统在疫苗研究中的应用进展

Progress in vaccine development based on baculovirus expression vector system

摘要:

昆虫杆状病毒表达载体系统 (Baculovirus expression vector system，BEVS) 已成功应用于多种蛋白的表达，并为疫苗开发提供了充足的原材料。相比其他表达系统，BEVS具有许多优势：杆状病毒专一寄生于无脊椎动物，安全性高；重组蛋白表达水平高；可对重组蛋白进行正确折叠和翻译后修饰，获得具有生物活性的蛋白；适应于多基因表达如病毒样颗粒 (Virus-like particle) 的复杂设计；适用于大规模无血清培养等。为了更好地理解BEVS在疫苗研究中的应用前景，文中将从BEVS的发展及其在疫苗研究中的应用等方面进行综述。

关键词:杆状病毒，昆虫细胞，蛋白表达，疫苗，病毒样颗粒

Abstract:

Baculovirus expression vector system (BEVS) has been successfully applied to the over-expression of various proteins, thus providing sufficient materials for vaccine research. Compared to other systems, BEVS has many advantages: baculovirus solely being parasitic in invertebrates, the resultant products conferring high safety to mammalian, high expression level of recombinant proteins, preferable folding for eukaryotic protein, proper post-translational modification required for biological function, suitable for multiple genes co-expression and large-scale production with serum-free culture media. To better understand the advantages and prospective of BEVS for the vaccine research, this article will review the development of BEVS and its application on vaccine research.

Key words:baculovirus, insect cell, protein expression, vaccine, virus-like particles