昆虫杆状病毒表达载体系统在疫苗研究中的应用进展

Progress in vaccine development based on baculovirus expression vector system

摘要:

昆虫杆状病毒表达载体系统 (Baculovirus expression vector system，BEVS) 已成功应用于多种蛋白的表达，并为疫苗开发提供了充足的原材料。相比其他表达系统，BEVS具有许多优势：杆状病毒专一寄生于无脊椎动物，安全性高；重组蛋白表达水平高；可对重组蛋白进行正确折叠和翻译后修饰，获得具有生物活性的蛋白；适应于多基因表达如病毒样颗粒 (Virus-like particle) 的复杂设计；适用于大规模无血清培养等。为了更好地理解BEVS在疫苗研究中的应用前景，文中将从BEVS的发展及其在疫苗研究中的应用等方面进行综述。

Abstract:

Baculovirus expression vector system (BEVS) has been successfully applied to the over-expression of various proteins, thus providing sufficient materials for vaccine research. Compared to other systems, BEVS has many advantages: baculovirus solely being parasitic in invertebrates, the resultant products conferring high safety to mammalian, high expression level of recombinant proteins, preferable folding for eukaryotic protein, proper post-translational modification required for biological function, suitable for multiple genes co-expression and large-scale production with serum-free culture media. To better understand the advantages and prospective of BEVS for the vaccine research, this article will review the development of BEVS and its application on vaccine research.