不同微生物大鼠腹腔注射模型尿液蛋白组学的比较

Comparison of urine proteome in rats intraperitoneally infected by different microorganisms

摘要:

不同的微生物都可以引起腹腔感染，文中尝试利用尿液来区分不同的微生物感染。通过在大鼠腹腔内分别注射大肠杆菌、金黄色葡萄球菌和白色念球菌建立3种模型，收集感染后0、12、36、72 h的尿液，并使用液相色谱串联质谱技术 (LC-MS/MS) 对尿蛋白进行分析。与感染前相比，在大肠杆菌腹腔注射模型中共鉴定到69个差异蛋白，在金黄色葡萄球菌腹腔注射模型中共鉴定到31个差异蛋白，在白色念球菌腹腔注射模型中共鉴定到38个差异蛋白。结果表明，腹腔注射不同的微生物时尿蛋白质组不同，提示尿液可能对不同的腹腔感染有鉴别诊断的潜能。

Abstract:

Different microorganisms can cause intraperitoneal infection. This study was to distinguish different microbial infections by urine analysis. Rats were intraperitoneally injected with Escherichia coli, Staphylococcus aureus, and Candida albicans, separately. Urine samples were collected from rats at 0, 12, 36 and 72 h after infection. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (without infection), a total of 69 differential proteins were identified in rats injected with E. coli. A total of 31 differences proteins were identified in rats injected with S. aureus. A total of 38 differential proteins were identified in rats injected with C. albicans. Urine proteome was different when rats were infected by different microorganisms, suggesting that urine may have the potential for differential diagnosis of different intraperitoneal infections.