森林草莓SUN基因家族的鉴定及其对逆境的响应

Genome-wide identification of SUN gene family in Fragaria vesca and stresses-response analysis

摘要:

SUN基因是调控植物生长发育的关键基因。本研究鉴定了二倍体森林草莓（Fragaria vesca）的SUN基因家族，并对各成员的理化性质、基因结构、系统进化以及基因表达进行了分析。结果表明，森林草莓有31个FvSUN基因，其编码蛋白可聚类为7个组，同一组内成员具有高度相似的基因结构与编码蛋白保守域；FvSUNs蛋白的亚细胞定位主要在细胞核中。共线性分析表明森林草莓FvSUNs基因家族主要通过染色体片段复制产生，拟南芥与森林草莓存在23对直系同源基因。利用森林草莓的转录组数据，对FvSUNs基因的组织表达特征进行分析，发现主要可归为3类：各组织均表达、组织中几乎不表达、组织特异性表达，并通过实时荧光定量PCR （quantitative real-time polymerase chain reaction，qRT-PCR）进一步验证结果。此外，还对森林草莓进行不同的逆境胁迫处理，qRT-PCR分析了31个FvSUNs基因的表达情况，发现大部分基因均在不同程度上受低温、高盐或干旱胁迫的诱导表达。这些研究结果为深入揭示草莓SUN基因的生物学功能及其分子机制奠定了基础。

Abstract:

SUN gene is a group of key genes regulating plant growth and development. Here, SUN gene families of strawberry were identified from the genome of the diploid Fragaria vesca, and their physicochemical properties, genes structure, evolution and genes expression were also analyzed. Our results showed that there were thirty-one FvSUN genes in F. vesca and the FvSUNs encoded proteins were classified into seven groups, and the members in the same group showed high similarity in gene structures and conservative motifs. The electronic subcellular localization of FvSUNs was mainly in the nucleus. Collinearity analysis showed that the members of FvSUN gene family were mainly expanded by segmental duplication in F. vesca, and Arabidopsis and F. vesca shared twenty-three pairs of orthologous SUN genes. According to the expression pattern in different tissues shown by the transcriptome data of F. vesca, the FvSUNsgene can be divided into three types:(1) expressed in nearly all tissues, (2) hardly expressed in any tissues, and (3) expressed in special tissues. The gene expression pattern of FvSUNs was further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the seedlings of F. vesca were treated by different abiotic stresses, and the expression level of 31 FvSUNs genes were assayed by qRT-PCR. The expression of most of the tested genes was induced by cold, high salt or drought stress. Our studies may facilitate revealing the biological function and molecular mechanism of SUN genes in strawberry.