酪醇及其衍生物的微生物代谢工程调控研究进展

Recent advances in metabolic engineering of microorganisms for production of tyrosol and its derivatives

摘要:

酪醇是一种天然酚类化合物，具有抗氧化、抗炎症等多种生物活性，是羟基酪醇和红景天苷等高价值天然产物的重要前体物质。近年来酪醇及其衍生物的绿色高效合成受到广泛关注，利用代谢工程改造微生物构建细胞工厂具有成本低且绿色环保等优点，成为最具潜力的产业化方式。本文介绍了酪醇的生物合成路径，主要针对大肠杆菌和酿酒酵母从头合成酪醇路径中的调控节点进行解析，综述了大肠杆菌和酿酒酵母合成酪醇的研究进展，并对其衍生物-羟基酪醇和红景天苷的代谢工程研究进展进行介绍，为构建酪醇及其衍生物高产工程菌株提供参考。

Abstract:

Tyrosol is a natural phenolic compound with antioxidant, anti-inflammatory and other biological activities, serving as an important precursor of high-value products such as hydroxytyrosol and salidroside. Therefore, the green and efficient biosynthesis of tyrosol and its derivatives has become a research hotspot in recent years. Building cell factories by metabolic engineering of microorganisms is a potential industrial production way, which has low costs and environmental friendliness. This paper introduces the biosynthesis pathway of tyrosol and presents the key regulated nodes in the de novo synthesis of tyrosol in Escherichia coli and Saccharomyces cerevisiae. In addition, this paper reviews the recent advances in metabolic engineering for the production of hydroxytyrosol and salidroside. This review can provide a reference for engineering the strains for the high-yield production of tyrosol and its derivatives.