基于工程化盐单胞菌TDZI-08一锅法合成衣康酸

One-pot synthesis of itaconic acid by engineered Halomonas bluephagenesis TDZI-08

摘要:

衣康酸(itaconic acid, IA)是12种高附加值平台化合物之一，广泛应用于涂料、黏合剂、塑料、树脂和生物燃料等领域。本研究基于前期构建的工程化盐单胞菌TDZI-08，通过排查阻碍因素、优化碳源、氮源、诱导剂添加时间和表面活性剂添加量等，建立了一锅法催化柠檬酸合成衣康酸工艺。在5 L发酵罐中利用TDZI-08进行开放式不灭菌一锅法合成，最高产生了40.50 g/L衣康酸，催化阶段得率为0.68 g衣康酸/g柠檬酸，总得率为0.42 g衣康酸/g (柠檬酸+葡萄糖酸)。本研究探索出的一锅法合成体系工艺简单且无须灭菌和无菌操作，表明盐单胞菌具有较好的衣康酸工业化生产潜力。

Abstract:

Itaconic acid (IA) is one of the twelve high value-added platform compounds applied in various fields including coatings, adhesives, plastics, resins, and biofuels. In this study, we established a one-pot catalytic synthesis system for IA from citric acid based on the engineered salt-tolerant bacterial strain Halomonas bluephagenesis TDZI-08 after investigating factors that hindered the process and optimizing the carbon source, nitrogen source, inducer addition time, and surfactant dosage. The open, non-sterile, one-pot synthesis with TDZI-08 in a 5 L fermenter achieved the highest IA titer of 40.50 g/L, with a catalytic yield of 0.68 g IA/g citric acid during the catalytic stage and a total yield of 0.42 g IA/g (citric acid+gluconic acid). The one-pot synthesis system established in this study is simple and does not need sterilization or aseptic operations. The findings indicate the potential of H. bluephagenesis for industrial production of IA.